A SPATIALLY SELECTIVE, FIXED-OPTICS MULTICOLOR FLUORESCENCE DETECTION SYSTEM FOR A MULTICHANNEL MICROFLUIDIC DEVICE, AND METHOD FOR DETECTION

摘要

A fixed-optics system for detecting fluorescence light emitted from one or more spatially selected volumes (S), e.g., in one or more microfluidic channels, comprises a detection optical fiber (60) corresponding to each selected volume and a ball lens (110) adjacent to one end of each optical fiber, wherein the ball lens has a diameter (D) larger than the diameter of the optical fiber and a distance (dl) between the ball lens and the optical fiber is in a range of 3 to 40 times the diameter of the optical fiber, so that due to a quasi-focusing configuration light emitted from the spatially selected volume and received by the ball lens converges onto the end (60a) of the optical fiber.