Method for diagnostic and therapeutic target discovery by combining isotopic and isobaric labels

摘要

The present invention describes isotopic and isobaric labelling reagents suitable for double labelling different individual protein samples. After double labelling of the individual protein samples, the labelled polypeptides in an individual sample have a unique combination of an isotopic and an isobaric label. Thereafter all samples are pooled. Peptides from the pooled samples are isolated and analysed by mass spectrometry for determining the relative concentration of each differentially labelled polypeptide