DISCOVERY, CLONING AND PURIFICATION OF THERMOCOCCUS SP. (STRAIN 9°N-7) DNA LIGASE

摘要

Compositions that describe a thermostable DNA ligase isolated from Thermococcus sp. (strain 9°N-7) and methods for making und using the same are described. The thermostable DMA ligase depends on ATP and not NAD+ as a cofactor during ligation, and retains activity at 100°C.