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GENETIC SELECTION SYSTEM TO IDENTIFY PROTEASES, PROTEASE SUBSTRATES AND PROTEASE INHIBITORS
GENETIC SELECTION SYSTEM TO IDENTIFY PROTEASES, PROTEASE SUBSTRATES AND PROTEASE INHIBITORS
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机译:鉴定蛋白酶,蛋白酶底物和蛋白酶抑制剂的遗传选择系统
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摘要
The present invention concerns a tester protein for identifying and/or monitoring protease activity in a cellular assay suitable for high throughput screenings by growth selection, wherein the tester polypeptide is a non-regulatory protein carrying a protease cleavage sequence. Upon co-expression of the protease recognizing said cleavage sequence the tester protein is inactivated, which influences the growth and/or survival of the host cells under the chosen conditions. However, in the presence of protease inhibitor the growth phenotype is reversed. The system can be used to identify proteases, protease inhibitors, and protease cleavage sites.
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