TRANSGENIC ZEBRAFISH FOR AN ASSESSMENT OF ENVIRONMENTAL CONTAMINANTS AND METHOD FOR PRODUCING THEREOF

摘要

It is arranged to evaluate effect and susceptibility before clinical use by providing a biomarker for the risk of pollutant present in ambient enviroment for searching for a transgenic zebrafish of pollutant present in ambient enviroment and preparation method. A kind of method, it is used to prepare the transgenic zebrafish for searching for pollutant present in ambient enviroment to include the following steps: :(a) a pollutant reaction gene promoter of human cloning, such as HSRE (thermal shock response element) and AhRE (aryl-hydrocarbon response element); (b) insertion auxiliary agent enters a vector one fluorescent protein of expression; (c) vector is introduced to zebra fish. One transgenic zebrafish for search for pollutant present in ambient enviroment by the method is characterized in that: it is radiated when it is exposed to environmental pressure, such as it is hot, cold, anoxic, Cd, L- azetidine -2- acid, 2,3,7,8-tetrachlorodibenzo-p- dioxin, 3-MECA, 3,4,5,3', 4', 5'- hexabromobiphenyl, chlorinated diphenyl 1254 and benzo [one] pyrene.