A kind of method is arranged to preparation with efficient carrageenan lyase by cultivating a new Pseudomonas sp for producing carrageenan lyase. HS5322 (depositions not. KCTC 18103P) be isolated with Rapana venosa. A kind of method includes the following steps: for producing carrageenan lyase :(a) is inoculated with to Pseudomonas sp. HS5322 (KCTC 18103P) into a fermentation tank includes culture medium comprising the 10g/l of carrageenan, the 10g/l of peptone, a yeast extract 10 g/l, the 750ml/l of seawater and the 250ml/l of distilled water; (b) with the 0. of offer air thereto1 vvm incubation steps (a) and in 20 deg.The 7. of a temperature of CThe agitation with 200 rev/min speed under 5 pH, 76 hours production carrageenan lyases, carrageenan lyase has 35 deg. hereThe 2. of an optimal reactive temperature of C, an optimal pH of 7-8 and a best NaCl activation0% concentration and 8.Stablize under 0 pH in 30-40 deg.A temperature of C.
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