A kind of method, entering cardiac muscle cell and be arranged to production for distinguishing mescenchymal stem cell derived from umbilical cord blood has efficient cardiac muscle cell, is used to require specially controlling for Myocardial Regeneration cardiopathic new, such as heart failure. A kind of method, enter cardiac muscle cell comprising steps of including regularly thereto by the BMP-2 protein of pressure change in a medium culture mescenchymal stem cell derived from umbilical cord blood, wherein a series of pressure change to low pressure from 5-30mmHg to 120-200mmHg highest pressures and medium further comprises TGF-β for distinguishing mescenchymal stem cell derived from umbilical cord blood.
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