SUBSTRATES FOR ANALYZING THE COVERAGE OF SELF-ASSEMBLED MOLECULES AND METHODS OF ANALYZING THE COVERAGE OF SELF-ASSEMBLED MOLECULES USING THE SAME

摘要

Substrates for analyzing the coverage of self-assembled molecules are provided to measure efficiently the presence and reaction degree of functional groups on the surface of self-assembled molecules by using nanoparticles without use of complicated methods such as FT-IR(Fourier Transform InfraRed), XPS(X-ray photoelectron spectroscopy) and fluorescence method. A substrate for analyzing the coverage of self-assembled molecules comprises: a substrate(100) for immobilizing biomaterials; a self-assembled molecule layer(102) formed on the substrate and having a functional group capable of reacting with an amine group; a capture DNA molecule(200) having the amine group to be combined with the self-assembled molecule layer; and a probe DNA molecule combining with the capture DNA molecule and having nanoparticles on the surface, wherein the functional group capable of reacting with an amine group is -SH, -NH2, -Si(OCH3)3, -Si(OC2H5)3 and -Si(Cl)3. Further, the substrate(100) for immobilizing biomaterial is one selected from a group consisting of glass, polycarbonate, polyester, polyethylene, polypropylene and wafer.