首页>
外国专利>
NOVEL CYCLODEXTRIN GLYCOSYLTRANSFERASE SPECIFIC FOR INTERMOLECULAR TRANSGLYCOSYLATION OF FUNCTIONAL ANTIOXIDANT BIOFLAVONOID FROM BACILLUS SP. BL-31 AND ITS THERMOSTABLE MUATANT ENZYME
NOVEL CYCLODEXTRIN GLYCOSYLTRANSFERASE SPECIFIC FOR INTERMOLECULAR TRANSGLYCOSYLATION OF FUNCTIONAL ANTIOXIDANT BIOFLAVONOID FROM BACILLUS SP. BL-31 AND ITS THERMOSTABLE MUATANT ENZYME
A novel alkalophilic Bacillus sp. BL-31 strain is provided to secret cyclodextrin glycosyltransferase which shows very high reaction activity on transglycosylation of a functional natural antioxidizing bioflavonoid. A cyclodextrin glycosyltransferase secreted by the novel strain is provided to show the transglycosylation enzyme characteristics for producing transglycosylated bioflavonoid. A novel alkalophilic Bacillus sp. BL-31 producing cyclodextrin glycosyltransferase showing specificity to transglycosylation of bioflavonoid is deposited as a deposition no. KACC 91294P. A cyclodextrin glycosyltransferase enzyme showing high specificity on transglycosylation of bioflavonoid consists of an amino acid sequence of SEQ ID : NO. 1 produced by the Bacillus sp. BL-31. A gene encoding the cyclodextrin glycosyltransferase consists of a nucleotide sequence described as SEQ ID : NO. 2 and is registered as a GeneBank nucleotide sequence database registration no. EF363797.
展开▼
机译:新型嗜碱芽孢杆菌提供BL-31菌株以分泌环糊精糖基转移酶,该酶对功能性天然抗氧化生物类黄酮的转糖基化显示非常高的反应活性。提供了由新菌株分泌的环糊精糖基转移酶,以显示用于产生转糖基化的生物类黄酮的转糖基化酶的特性。新型嗜碱芽孢杆菌产生对生物类黄酮的转糖基化具有特异性的产生环糊精糖基转移酶的BL-31以沉积号No.1沉积。 KACC 91294P。对生物类黄酮的转糖基化显示高特异性的环糊精糖基转移酶由氨基酸序列SEQ ID NO:1组成。 1由芽孢杆菌属产生。 BL-31。编码环糊精糖基转移酶的基因由描述为SEQ ID NO:1的核苷酸序列组成。序列号为2,并注册为GeneBank核苷酸序列数据库注册号。 EF363797。
展开▼