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Ubiquitous promoter primers for amplification thereof the expression vector comprising the same and the transformation plant

机译:用于扩增的遍在启动子引物,包含所述引物的表达载体和转化植物

摘要

A ubiquitous promoter is provided to be able to induce the systemic expression of Arabidopsis thaliana as well as other crops when modified by a plant genetic engineering method, thereby being used for expressing a useful gene, a marker or a reporter gene or improving agricultural characteristics. A ubiquitous promoter is isolated from Arabidopsis thaliana and consists of SEQ ID : NO. 1. A primer for amplifying the ubiquitous promoter consists of an oligonucleotide consisting of SEQ ID : NOs. 6 and 7. A transgenic plant is transformed by an expression vector including the ubiquitous promoter, where the plant is a root vegetable such as carrot, sweet potato and radish, a medicinal plant such as ginseng and Codonopis lanceolata, a forage crops such as corn, and staple grains such as rice.
机译:提供了一种普遍存在的启动子,当通过植物基因工程方法进行修饰时,能够诱导拟南芥以及其他农作物的系统表达,从而用于表达有用的基因,标记或报告基因或改善农业特性。遍在启动子是从拟南芥中分离的,并由SEQ ID NO:1组成。 1.用于扩增遍在启动子的引物由由SEQ ID NO:1组成的寡核苷酸组成。参照图6和图7。用包括遍在启动子的表达载体转化转基因植物,其中该植物是根菜,例如胡萝卜,红薯和萝卜,药用植物,例如人参和党参(Condonopis lanceolata),草料作物例如玉米。以及大米等主食。

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