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Ubiquitous promoter primers for amplification thereof the expression vector comprising the same and the transformation plant
Ubiquitous promoter primers for amplification thereof the expression vector comprising the same and the transformation plant
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机译:用于扩增的遍在启动子引物,包含所述引物的表达载体和转化植物
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摘要
A ubiquitous promoter is provided to be able to induce the systemic expression of Arabidopsis thaliana as well as other crops when modified by a plant genetic engineering method, thereby being used for expressing a useful gene, a marker or a reporter gene or improving agricultural characteristics. A ubiquitous promoter is isolated from Arabidopsis thaliana and consists of SEQ ID : NO. 1. A primer for amplifying the ubiquitous promoter consists of an oligonucleotide consisting of SEQ ID : NOs. 6 and 7. A transgenic plant is transformed by an expression vector including the ubiquitous promoter, where the plant is a root vegetable such as carrot, sweet potato and radish, a medicinal plant such as ginseng and Codonopis lanceolata, a forage crops such as corn, and staple grains such as rice.
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机译:提供了一种普遍存在的启动子,当通过植物基因工程方法进行修饰时,能够诱导拟南芥以及其他农作物的系统表达,从而用于表达有用的基因,标记或报告基因或改善农业特性。遍在启动子是从拟南芥中分离的,并由SEQ ID NO:1组成。 1.用于扩增遍在启动子的引物由由SEQ ID NO:1组成的寡核苷酸组成。参照图6和图7。用包括遍在启动子的表达载体转化转基因植物,其中该植物是根菜,例如胡萝卜,红薯和萝卜,药用植物,例如人参和党参(Condonopis lanceolata),草料作物例如玉米。以及大米等主食。
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