ANTIOXIDANT EXTRACT OF FERMENTED MUSHROOM CYATHUS STERCOREUS, ANTIOXIDANT COMPOUNDS ISOLATED THEREFROM AND A METHOD FOR PREPARATION OF THE SAME

摘要

Novel compounds isolated from an extract of fermented mushroom Cyathus stercoreus are provided to show 2,2'-azinobis-(3-ethyl-benzothiazoline-6-sulfonic acid) radical(ABTS) and 1,1-diphenyl-2-picryl-hydrazyl radical(DPPH) scavenging function. An extract with antioxidizing activity is obtained by extracting an extract of fermented mushroom Cyathus stercoreus with at least one organic solvent selected from the group consisting of chloroform, ethylacetate and butanol. A compound having free oxygen scavenging function is represented by the formula(1), wherein R is H, OH, OCH(CH3)2 or OCH3. A composition for scavenging free oxygen comprises the fermented mushroom Cyathus stercoreus, the extract derived from the fermented mushroom Cyathus stercoreus or the compound of the formula(1) as an effective ingredient. A method for preparing the compound of the formula(1) comprises the steps of: (a) obtaining the extract with antioxidizing activity; (b) respectively extracting the extract with chloroform and methanol; and (c) recrystallizing the methanol extract or purifying the chloroform extract through reverse column chromatography, silica gel column chromatography, and pre-HPLC in sequence.