HYALURONIC ACID NANOGELS FOR PHYSICAL ENCAPSULATION OF MACROMOLECULAR DRUGS AND FABRICATION METHOD THEREOF

摘要

A method for preparing a hyaluronic acid nanogel is provided to encapsulate a macromolecular drug in a hyaluronic acid nano-gel physically and provide a drug delivery system with excellent drug delivery rate in vivo, stability, drug activity and excellent selectivity on a specific receptor of cancer cells. A method for preparing a hyaluronic acid nanogel containing a therapeutic drug comprises the steps of: (a) dissolving the drug such as plasmid gene, antisense ODN, siRNA, RNA, peptide, protein and water-soluble drug and a hyaluronic acid where a free thiol group is introduced into a carboxylic acid in an aqueous solution; (b) adding the aqueous solution to an organic solvent such as methylene chloride, chloroform, benzene, toluene and hexane to form a W/O reverse emulsion and (c) auto-crosslinking the free thiol group through disulfide bond. Further, a molecular weight of the hyaluronic acid is 10,000 to 2x10^7, and a diameter of the hyaluronic acid nanogel is 100 to 900nm.