Transformed E.coli producing antihypertensive peptide and the method for mass producing recombinant antihypertensive peptide using the same

摘要

A method for mass-producing a recombinant antihypertensive peptide is provided to be usefully used for producing a peptide having low molecular weight at low cost with high efficiency and use the antihypertensive peptide for a recombinant expression system for foods. A method for preparing a recombinant pEUH40 having a cleavage map depicted in Fig. 2 comprises the steps of: (a) inserting a gene of SEQ ID : NO. 1 into a cloning vector pUC18; (b) after self-ligating the gene of SEQ ID : NO. 1, inserting the self-ligated gene into the recombinant vector obtained from the step(a) to transform E. coli DH5alpha; and (c) after inserting the vector obtained from the step(b) into a vector pET29b, fusing a ubiquitin gene thereinto. A method for mass-producing a recombinant antihypertensive peptide His-His-Leu comprises the steps of: (a) culturing 40 copies of His-His-Leu sequences using a transgenic E.coli KCTC 11004BP including the recombinant pEUH40 plasmid; (b) after centrifuging the culture material, eluting cells therefrom and extracting and purifying a cell eluent with cation exchange chromatography; and (c) after hydrolyzing the purified material with leucine aminopeptidase, isolating His-His-Leu from the hydrolysate through HPLC.