Methods for detecting a target gene using a peptide nucleic acid probe immobilized microarray

摘要

A method for detecting a target gene using a peptide nucleic acid probe immobilized microarray is provided to re-use an already constructed microarray without constructing new microarrays for different target genes by performing PCR after linking a nucleotide sequence complementary to a nucleotide sequence of a peptide nucleic acid(PNA) as an addressor to a primer for amplifying the target gene and detecting the corresponding nucleotide sequence from the obtained PCR product. A method for detecting a target gene using a peptide nucleic acid probe immobilized microarray comprises the steps of: (a) immobilizing a PNA having 5-30 nucleotide sequences on a solid support for microarray to prepare a PNA-probe immobilized microarray; (b) coupling a nucleotide sequence complementary to the nucleotide sequence of the PNA to a 5'-terminal of a forward primer for amplifying the target gene; (c) coupling at least one fluorescent material to a reverse primer for amplifying the target gene; (d) subjecting a nucleic acid sample obtained from a test body to polymerase chain reaction(PCR) using the forward primer and the reverse primer respectively obtained from the steps(b) and (c) to obtain a PCR product; and applying the PCR product to the microarray obtained from the step(a) to detect the existence of the target gene, wherein the target gene is a TRAF-1(TNF receptor associated factor-1) gene selected from the group consisting of polynucleotides of SEQ ID ; NOs. 1 to 3 and the PNA has nucleotide sequences of SEQ ID : NOs. 4 to 8. Further, the target gene comprises a single nucleotide polymorphism or a mutation.