E. coli Having Organophosphorus Hydrolase Expressed and Secreted into Periplasmic Space

摘要

The invention that is the organophosphorus hydrolase is expressed and secreted into the cell gap to contain an organophosphorus hydrolase recombinant expression vector, the coding sequence is operably linked to a control sequence including the secretory sequence or secretory sequence of TorA of PelB of characterized in E. coli, and the coding sequence of the organophosphorus hydrolase is constructed a recombinant expression vector operably linked to control sequences including secretory sequence or secretory sequence of TorA of PelB, and that; To transform Escherichia coli host cell with the recombinant expression vector, and; The transformed E. coli host cells by culturing the organophosphorus hydrolase is an organophosphorus hydrolase on the cell gap that allows expression and secretion to the cell gap is related to a method for producing Escherichia coli is expressed and secreted. ; In addition, the present invention in an organic hydrolysis coding sequence is PelB secretion sequence or TorA secretory sequence the organophosphorus hydrolase contain a recombinant expression vector operably linked to regulatory sequences, including of the enzyme is cell gap Expression and by contacting the waste water containing the E. coli and organophosphorus it characterized in that the secretion relates to a method for the removal of the organic waste water. ; Organophosphorus hydrolase, secretory sequence, a cell gap of E. coli