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Anther-specific genes derived from Malus x domestica promoters thereof and a method for preparing transformed plant using the same
Anther-specific genes derived from Malus x domestica promoters thereof and a method for preparing transformed plant using the same
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机译:源自苹果属(malus x domestica)启动子的花药特异性基因及其制备转化植物的方法
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摘要
The present invention relates to a drug specific gene, a promoter thereof, and a method for producing a transgenic plant using the same. Specifically, SEQ ID NOs: 1 and 2 which are specifically expressed only in drugs derived from apples ( Malus x domestica cv. Fuji) plants And promoters of the genes PMdASG1 , PMdASG2, andPMdASG3 , including the novel genes MdASG1 , MdASG2, andMdASG3, and some or all of which exhibit the transcriptional activity of SEQ ID NOs: 4, 5, and 6, respectively; It is about. The drug specific expression genes of the present invention can be usefully used to identify pollen development mechanisms involved in the fertilization of plants, and the promoters PMdASG1, PMdASG2 and PMdASG3 of the present invention express genes of interest only in pollen to plants or drugs. Since it shows high activity in specific expression for the development, it can be usefully used for the development of male infertile transgenic plants and the development of genetically modified plants to which foreign genes requiring pollen specific expression have been introduced.;Drug Specific Expression, Gene, Promoter, Male Infertility, Transgenic Plant
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机译:本发明涉及药物特异性基因,其启动子以及使用该基因生产转基因植物的方法。具体而言,仅在衍生自苹果( Malus I> x domestica I> cv。Fuji)植物的药物和基因的启动子中特异性表达的SEQ ID NOs:1和2。 PMdASG1 I>, PMdASG2和 I> PMdASG3 I>,,包括 I>新基因 MdASG1 I>, MdASG2,和 i> MdASG3 i>,并且其中一些或全部分别显示出SEQ ID NO:4、5和6的转录活性;关于。本发明的药物特异性表达基因可用于鉴定植物受精的花粉发育机制,本发明的启动子PMdASG1,PMdASG2和PMdASG3仅在花粉中向植物或药物表达目的基因。由于它在发育中的特异性表达中显示出高活性,因此可用于雄性不育转基因植物的发育和已引入需要花粉特异性表达的外源基因的转基因植物的开发。 ,启动子,男性不育,转基因植物
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