Screening method for the anti-allergic agents using M2-type pyruvate kinase assay

摘要

The present invention mast cells or mast cells of Beit Lou Pai kinase M _2(M 2 type Pyruvate kinase assay) by the use of anti-allergic substances in the way that the search is on. ; How to use the M ₂ PK search for new anti-allergic material is very simple, because the actual number to be applied through a simple enzyme activity measuring method or phosphorylation assay, economical and, each of the signaling elements involved in the signaling pathway of the IgE receptor a comprehensive and can be reflected in the overall effect to block, to be able to differentiate non-specific action on the allergic reaction and the cells, and provides a new method of searching for an optional anti-allergens in the IgE receptor signaling pathway.; IgE receptor kinase Beit Lou Pai, M₂PK, phosphorylation, anti-allergic drug search methods