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NEW nucleic acid sequences and their use in a way to achieve pathogen resistance in plants

机译:新的核酸序列及其在植物中实现病原体抗性的方式中的用途

摘要

1. A method for increasing the resistance to penetrating through mezofillovye cells pathogens in a plant or in the organ, tissue or cell, wherein the activity of callose synthase in a plant or in the organ, tissue or cell is reduced compared to control rasteniyami.2. A method according to claim 1, characterized in that the pathogens selected from the families comprising Pucciniaceae, Mycosphaerellaceae and Hypocreaceae.3. A method according to claim 1, characterized in that the lower the protein activity of callose synthase comprising the sequences shown in SEQ ID No: 2, 4, 6, 8, 10, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 or a protein having at least 40% homology to posledovatelnostyam.4 data. A method according to claim 1, characterized in that available in the plant, plant organ, tissue or cell activity of callose synthase is reduced so that the reduced activity of at least one polypeptide which is encoded by a nucleic acid molecule comprising at least one nucleic acid molecule from the group consisting of: a) nucleic acid molecule encoding a polypeptide comprising presented in SEQ ID No: 2, 4, 6, 8, 10, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35 sequence; b) nucleic acid molecule which comprises, at least one polynucleotide sequence shown in SEQ ID No: 1, 3, 5, 7, 9, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32 or 34; c) a nucleic acid molecule encoding a polypeptide whose sequence has at least 40% identity to the sequences SEQ ID No: 2, 4, 6, 8, 10, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33 or 35; d) a nucleic acid molecule of (a) to (c), which encodes a fragment or an epitope sequences according to SEQ. ID No .: 2, 4, 6, 8, 10, 11, 13, 1
机译:1。一种增加对植物或器官,组织或细胞中的甲氧充盈细胞病原体穿透的抗性的方法,其中与对照rasteniyami相比,植物或器官,组织或细胞中的愈伤组织合酶的活性降低。 2。 2.根据权利要求1所述的方法,其特征在于,所述病原体选自:伞形科,霉菌科和伞形科。3。 2.根据权利要求1的方法,其特征在于,包含SEQ ID No:2,4、6、8、10、11、13、15、17、19、21、23的序列的sequences糖合酶的蛋白质活性较低。 ,25、27、29、31、33或35或与posledovatelnostyam.4数据具有至少40%同源性的蛋白质。 2.根据权利要求1所述的方法,其特征在于,降低了call糖合酶在植物,植物器官,组织或细胞中可用的活性,使得由包含至少一种核酸的核酸分子编码的至少一种多肽的活性降低。选自以下的酸分子:a)编码包含SEQ ID No:2、4、6、8、10、11、13、15、17、19、21、23、25、27的多肽的核酸分子,29、31、33或35序列; b)核酸分子,其包含至少一个SEQ ID No:1、3、5、7、9、12、14、16、18、20、22、24、26、28、30、32中所示的多核苷酸序列或34; c)编码其序列与SEQ ID No:2、4、6、8、10、11、13、15、17、19、21、23、25的序列具有至少40%同一性的多肽的核酸分子, 27、29、31、33或35; d)(a)至(c)的核酸分子,其编码根据SEQ.ID.No.1的片段或表位序列。身份证号码:2,4,6,8,10,11,13,1

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