METHOD FOR PROMOTION OF ACTIVE CONFORMATION OF GLYCOSYLATED RECOMBINANT PROTEIN, METHOD FOR PREPARING ACTIVE GLYCOSYLATED PROTEIN AND METHOD FOR PREPARING COMPOSITION OF THIS PROTEIN FOR ADMINISTRATION TO USER AND/OR PATIENT OR FOR USING BY USER AND/OR PATIENT

摘要

FIELD: medicine, biotechnology, pharmaceutical industry.;SUBSTANCE: method involves increasing part of the most active conformation of a glycosylated recombinant protein secreted by mammalian cell by its contact with a reagent for coupled oxidation-reduction. The proposed promotion method of the most active conformation of protein is used in a method for preparing a glycosylated recombinant protein in its the most active conformation. Configuration isomer of protein prepared by the indicated method for preparing a glycosylated recombinant protein in it's the most active conformation used in a method for preparing the protein composition for its administration to user and/or a patient or for consumption by user and/or patient. Using of the proposed invention provides enhancing activity of glycosylated protein prepared by a method of recombinant DNAs using a mammalian cell.;EFFECT: improved preparing method, valuable properties of protein.;27 cl, 9 dwg, 2 tbl, 3 ex