ORGANIZATION OF CELLS SIMILAR TO TISSUE AND MACROSCOPICAL DESIGNS SIMILAR TO TISSUE OBTAINED BY MEANS OF CULTURE OF MACROMASS OF CELLS AND METHOD OF MACROMASS CULTURE

摘要

FIELD: medicine.;SUBSTANCE: method of obtaining the live organization of cells of a similar tissue is revealed, that is the macromass culture including three-dimensional designs similar to tissue, without the aid of a skeleton or a foreign matrix. The cells are plated in a vessel for cell culture with high density per unit area in a range from 3.33×10⁵ to 3×10⁶ cells per cm². The three-dimensional organization of the cells received in the specified way similar to a tissue is described. When cultivating cell macromasses it is possible to receive the cells, organized in forms similar tissues without the aid of a matrix, and three-dimensional macroscopical structures similar to tissue. In the given work the organization similar to a tissue and macroscopical structures similar to tissue are obtained from fibroblasts of a skin, the osteogene cells received from fat stromal cells, chondrocytes and from osteoblasts. Thus any skeleton or a foreign matrix for obtaining of a tissue or any other agents is not used (except high density of cell plating) which promote formation of a tissue, tissues have completely cellular parentage.;EFFECT: present invention allows simplifying the method of obtaining of substitutes of a human body tissue.;13 cl, 24 dwg, 1 tbl