A new probe and their use in bioaffinity - analyses

摘要

Homogeneous bioaffinity - examination with the steps of:a) providing a labeled probe with(i) a tr - fret - marking pair with a luminescent energy donor with the excited condition with a long service life and a luminescence acceptor with the excited state with low service life; and(ii) a reactive area, which is capable of performing a bioaffinity - component to be determined to detect;b) contacting the labeled probe with a sample from which is expected that these the bioaffinity - component;c) make it possible for the probe and the bioaffinity - component perform a detection reaction; wherein the probe, a different energy transfer efficiency in respect to the donor and the acceptor, when a reaction with the bioaffinity - component takes place, in comparison to a, not reacting probe;d) measuring an energy transfer - based signal from the acceptor of the reacted sample;e) measuring an energy transfer - based signal from the acceptor a unreacted probe in a reference examination;f) comparing the in the steps d) and e) obtained..