A method for the detection of bacteria in from the blood samples derived with the aid of a flow cytometry - analysis

摘要

A method for the detection of bacteria in derived from blood samples, comprisinga) of from blood samples derived,b) a sterile removal of aliquots of the derived from blood samples under laminar - flow - conditions,c) adding the aliquots from b) to a nutrient broth - solution comprising meat peptone, casein - peptone, yeast extract, nacl and glucose, with a ph of - value of 6,8 to 7,8,d) of incubation at 30 to 40°C. for at least 2 hours,e) centrifugation of the incubated nutrient broth - solution and discarding of the supernatant,f) reconstitution of the pellets from e) under laminar - flow - conditions in a pbs - solution,g) removal of aliquots from the reconstituted pellets from f) and addition of the equivalent to a buffer - solution, the thiazole orange, and incubation for a few minutes, andh) flow cytometric determination of the with thiazole orange marked bacteria in the incubated aliquots of g).