Sensitivity increasing process for HIV detection involves mixing biological samples, heating to over 60 degrees C, remixing with binder surfaces and removing HIV-RNA eluate

摘要

The sensitivity increasing process includes making available biological samples, a solution of chaotropic salts and surfaces binding nucleic acids. The samples are mixed with the solution and heated to over 60[deg] C, then with the binding surfaces to produce complexes. The unbonded nucleic acids are washed out and the HIV-RNA is eluted at up to 60[deg] C. The eluate is left standing and then heated to over 70[deg] C, amplifying the HIV-RNA, which can then be detected.