DNA sequence coding for an Aspergillus fumigatus phospholipase, e.g. useful for desliming vegetable oil

摘要

DNA sequence (I) coding for an Aspergillus fumigatus phospholipase (II) is new. DNA sequence (I) having SEQ ID NO:1 (a defined sequence of 2822 nucleotides given in the specification) and coding for a phospholipase (II) with SEQ ID NO:2 (a defined sequence of 633 amino acids given in the specification) is new. Independent claims are also included for: (1) expression construct comprising (I) linked to regulatory sequences for expression in a host cell; (2) recombinant host cell transformed with the expression construct; (3) plasmid B11B1Hind6 (DSM 18369); (4) phospholipase encoded by the coding portion (especially nucleotides 530-2388) of (I); (5) producing (II) by culturing a host cell as above; (6) desliming vegetable oil by adding an aqueous solution containing (II), heating at 30-70[deg] C for 1-12 hours and separating the aqueous and oil phases.