An expression system for antibiotics free production of polypeptides

摘要

The present invention relates to an expression system for the production of one or more target polypeptide / target polypeptides, comprising a host cell, in the genome of the dna - sequence, the glycerol - 3 - phosphate dehydrogenase, are inactivated or partially or completely is deleted and the with an extrachromosomal element, which - a dna sequence encoding the or the target polypeptide (e) and glycerol - 3 - phosphate dehydrogenase, has been transformed, wherein both the host cell genome as well as the extrachromosomal element no antibiotic resistance gene, and - a dna sequence encoding a polypeptide with glycerol - 3 - phosphate dehydrogenase activity, characterized in that the dna - sequence is selected from a) - dna sequences, a nucleotide sequence as shown in seq id no: 1, b) dna - sequences which a nucleotide sequence is reproduced by the nucleotides 1338 to 2375 of seq id no: 1, c) dna -, the sequences of the plasmid ptp01 with the plasmid map according to fig. 4 and deposited under deposit number dsm 18890 be coded, d) - dna sequences coding for the protein sequence of seq id no: 2, e) dna - sequences, the under stringent conditions with one of the dna - sequences according to (a), b), c) or d) hybridize, f) dna - sequences, which as a result of the degeneracy of the genetic code with the dna - / nucleotide sequences according to (a), b), c), d) or e) are used, and g) complementary strands to the sequences according to (a) to f), and the ..