Fluorescence imaging, must by means of telecentric stimulation - and imaging optics

摘要

The present invention relates to the field of DNA analysis. In particular, the present invention is directed to a device for the parallel imaging of fluorescence intensities at a plurality of sites as a measure for DNA hybridization. More particular, the present invention is directed to a device to image multiplex real time PCR or to read out DNA microarrays.;According to a preferred embodiment the device comprises holding means (1) for holding a planar support (2) with an assembly of multiple individual sites (3); at least one light source (4) for emitting light comprising at least one excitation frequency; a transducer (5) for receiving fluorescence signals from said sites; a field lens (6); an excitation lens arrangement (10), and an imaging lens arrangement (11), wherein said excitation light path and said fluorescence imaging light path are telecentric on the object side of said field lens.