Procedure for the manufacture of an oil containing long-chain, repeatedly unsaturated fatty acids from biomass, foods, food composition, cosmetic or pharmaceutical composition containing this oil

摘要

A method of preparation of a stable oil containing long-chain polyunsaturated fatty acids in the form of triacylglycerols by dry pressing one or more biomass deriving from the culture of a microorganism to obtain a first pressed oil and a cake and treating the oil with an absorbent and deodorizing in moderate conditions. The triacylglycerols are especially arachidonic acid (ARA), dihomogammalinoleic acid (DHGLA), docosahexenoic acid (DHA) and eicosapentenoic acid (EPA). The biomass is especially from the culture of a fungus or microalga. The pressed cake is contact with a support oil forming part of an alimentary, nutritional, pharmaceutical or cosmetic composition to transfer remaining long-chain fatty acids. The oil is separated, filtered and mixed with the first oil and refined under moderate conditions. Physical refining with the aid of a process agent is carried out during contact with the support oil or after obtaining the pressed oil. The pressed cake may be ground in the presence of the support oil under gentle conditions at a moderate temperature under an inert gas atmosphere.