method for treating biopolymer, microorganisms, or other materials with more than one type of magnetic particles is used.

摘要

The method of processing biopolymers, microorganisms and substances by using two or more kinds of magnetic particles according to this invention can automatically and consistently perform a series of processes, such as extraction, isolation or measurement of DNA and the like, by a combined use of at least two or more kinds of magnetic particles and can also prevent cross-contamination completely. The processing method of this invention is performed in the following manner. Biopolymers, microorganisms or substances are bound to magnetic particles by using a pipette tip removably mounted to the front end of a pipette nozzle of a transfer pipette to perform a purification process such as capturing of cells or dissolving of cell nuclei or protein to extract DNA and the like. The extracted DNA and the like are amplified as required and then particular DNA and the like are isolated by binding them, through the use of the pipette tip, to other magnetic particles that are coated with antibody, probe, biotin or streptavidin. Then, the isolated particular DNA and the like are assayed by chemiluminescence, fluorescence or coloring reaction. IMAGE