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Native passed chemical ligation of unprotected or n - cysteine - protected peptides in aqueous solutions
Native passed chemical ligation of unprotected or n - cysteine - protected peptides in aqueous solutions
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机译:天然通过化学连接的未保护或n-半胱氨酸保护的肽在水溶液中
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摘要
The present invention provides methods, apparatus and kits for synthesizing assembled peptides and proteins on a solid phase with sequential ligation of three or more unprotected peptide segments using chemoselective and mild ligation chemistries in aqueous solution. Also provided are methods of monitoring solid phase sequential ligation reactions using MALDI or electrospray ionization mass spectrometry of reaction products.
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