RECOMBINANT HYPERACTIVE TRANSPOSITION SYSTEMS DERIVED FROM TRANSPOSON MOS-1

摘要

The present invention relates to a hyperactive recombinant transposition system derived from the Mos-1 transposon, comprising at least the two following partners: a) a Mos-1 pseudo-transposon in which an exogenous nucleotide sequence of interest replaces the nucleotide sequence encoding the original Mos-1 transposase andb) a Mos-1 transposase provided in trans of said pseudo-transposon, at least one of said partners being genetically modified in an appropriate manner to improve the frequency of transposition of said exogenous nucleotide sequence of interest In addition to such systems, the invention provides hyperactive Mos-1 pseudo-transposons, hyperactive Mos-1 transposases, kits.The invention also relates to the use of one or more of the foregoing means for performing transpositions of sequences, and more particularly gene transfer, effective.