In vitro measurement of thrombomodulin functional activity, useful to detect increase in thrombomodulin rate associated with symptoms e.g. diabetes, comprises e.g. assay of protein C activation, adding plasma agent

摘要

Method of in vitro measurement of functional activity of thrombomodulin, comprises assay of protein C activation in protein C activated (PCa) by thrombin in the presence of its cofactor (thrombomodulin), in a biological sample and in a medium; adding plasma agents needed in protein C activation system, in sample; and adding purified protein C and a fibrin polymerization inhibitor. Independent claims are included for: (1) a kit for measuring the functional activity of the thromobomodulin in biological medium, preferably plasma, comprising a first reagent comprising thrombin diluted in a buffer comprising a fibrin polymerization inhibitor and calcium, a second reagent comprising purified protein C, optionally a third reagent comprising an enzymatic substrate of the activated protein C; and (2) a method of in vitro detection of abnormal clotting comprising measuring of the plasmatic thrombomodulin functional activity.