Real-time linear birefringence detection polarizing microscope

摘要

The capture real-time polarization microscope images to quantify the LB signal detected where discrete polarization microscope image, various embodiments of the present invention is directed to the output and analysis. In one embodiment of the invention, circularly polarized light is passed into the sample and is imaged optically with polarized light microscopy conventional components. Thereafter, the analysis sub-four images which are analyzed by four-way separated by the analyzer or four-way polarizer, the resulting image obtained as a result, the original that has been generated by light microscopy imaging components of a conventional linear birefringence of each pixel at each position within the representation of the size of a quarter of the image, the extinction angle and, calculated processed to generate pseudocolor three representing the transmission, real-time image. Pseudo color images can be produced at a rate of 30 frames or more per second by using the calculation processing of the captured image by the programming techniques and efficient high imaging efficiency.