The invention describes the manufacture and use of secretory cell containing bead structures that are coated with agarose. The beads, which are preferably 4 mm-12 mm in diameter, and which preferably contain islets, are made of a particular agarose, i.eagarose which has a sulfate content of less than 0.2 wt % but greater than zero, a pyruvate content of 0-0.1 wt %, and a Kjeldahl nitrogen content of 0-0.04 wt %. The gels found from the agarose exhibit a gel strength of at least 1200 g/cm2 (1.0 wt % concentration), substantial absence of DNA binding in 0.07 M or less tris acetate buffer, and an EEO at 1.0 wt % agarose concentration of 0.05 or less.
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机译:本发明描述了用琼脂糖包被的含分泌细胞的珠结构的制造和使用。优选地,直径为4mm-12mm并且优选地包含胰岛的珠子由特定的琼脂糖,即蔗糖制成,其硫酸盐含量小于0.2wt%但大于零,丙酮酸盐含量为0。 0-0.1重量%,凯氏氮含量为0-0.04重量%。从琼脂糖中发现的凝胶显示出至少1200 g / cm2(1.0 wt%的浓度)的凝胶强度,在0.07 M或更少的tris乙酸盐缓冲液中基本不存在DNA结合,在1.0 wt%的琼脂糖浓度为0.05或0.5时的EEO减。
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