Use of intracellular localization profile as a predictive indicator or diagnostic indicator

摘要

By immunohistochemical techniques, the method of the present invention relates to the measurement of signal transduction activity in tissue sections. In addition to the expression level of the effector molecules of one or more receptor signaling pathway, the expression level of the receptor of interest is measured. Furthermore, the combined ratio of the expression level of the effector molecule in the cell compartment and the expression of the receptor, may have importance in the prognosis revealed. In one embodiment, reagents for detecting cell receptor protein 1) target, the method steps to stain tissue sections using the reagents, for detecting signaling effector molecule of at least one 2) , and a procedure as well as to analyze the stained sections, to quantify the effector molecules and receptor proteins of relative amounts.