Method and the device which find the central amplitude of the pulse which displays the grain size which is formed by the sizing system which is based on the aperture

摘要

Pipelined digital processing circuitry for use with a particle size measurement system, such as that employed in automated hematology systems, measures the center amplitude of a pulse produced by a particle or cell passing through a flow cell measurement aperture, such as the detection aperture of a Coulter Principle-based electronic particle analysis system. The circuitry of the invention processes successive pulse samples by means of a half-peak/half-width methodology that analyzes each pulse as its continuously sampled and temporarily stored in memory. Concurrent analysis of the data in memory during storage locates the pulse width at a prescribed percentage (e.g., 50%) of the peak amplitude of the pulse. This pulse width data is then processed to determine the pulse amplitude at the midpoint of the width of the pulse between its mid-peak values on rising edge and fall edge portions of the pulse.