The methodological null 8 amino 7 keto pelargonic acid which gets over the obstacle

摘要

PROBLEM TO BE SOLVED: To provide a method for bio-synthesizing a biotin vitamer in which the conversion from KAPA to DAPA is not obstructed due to the supply of a high concentration of an amino donor. ;SOLUTION: This method for overcoming the obstacle by a DAPA aminotransferase in biotin vitamer, which is a method for producing a biotin vitamer, is provided by (a) culturing bacteria having a DAPA aminotransferase utilizing lysine in an environment rich in lysine, a lysine analog or a lysine precursor, or (a) culturing bacteria having a DAPA aminotransferase utilizing lysine and being de-adjusted in regard to the production of lysine, and (b) recovering the biotin vitamer. The bacteria are engineered so as to produce the DAPA aminotransferase utilizing lysine and the DAPA aminotransferase utilizing SAM excessively, and especially the bacteria are further engineered so as to produce the biotin vitamer excessively by a de-adjustment caused by a bio A expression and in addition by an engineering at least one biotin synthesizing process.;COPYRIGHT: (C)1999,JPO