rianojinreseputatanpaku of kaiko, the cDNA, rearrangement vector, revelation plasmid, stationary revelation cell, screening method of insecticidal component and screening method of rianojinreseputa reactant of kaiko

摘要

In order that rianojinreseputatanpaku and the cDNA of kaiko are elucidated, screening method of the new insecticidal component is established, amino acid arrangement of the RyR protein of kaiko which so far is not yet elucidation, and that the cord/code cDNA which is done (1) base arrangement was made clear. In addition, sub cloning doing same cDNA (1) in the plasmid, that it draws up the revelation plasmid (3), furthermore draws up the RyR gene stationary revelation cell of kaiko, introduces this revelation plasmid into the animal cell, you say it does the Ca²⁺ response reaction inside the cell for the suffering reagent making use of the stationary revelation cell with fluorometry screening method of the insecticidal component was constructed.