Peptide ester reagents, and used for the preparation of the thioester compound or its ligation

摘要

How to ligation by an amide bond (amino acid, peptide) and the component proteins or by using a peptide ester as the raw material, the invention relates to reagents and are suitable for use in the method. The present invention provides a method for producing a thioester compound with a peptide ester. Following formula as a reagent to be used for ligation [Formula 1] (In the formula, X-CO-, may be modified, it is the residue of a peptide or protein, consisting of amino acids from two or more amino acids, Cys is a cysteine ​​residue, R ₂ is also be modified It is possible to increase the peptide ester shown in.) shows the amino acid residues may, -OH of the carboxyl group has been replaced.