Being nuclear acid fixation manner in order to be fixed nuclear acid fixation manner

摘要

A superior adsorption method for immobilizing nucleic acid probe on the surface of a solid phase substrate is provided. A nucleic acid immobilization method for immobilizing nucleic acid on a solid phase substrate comprising: bringing the above-mentioned solid phase substrate into contact with a composition comprising a total concentration of 0.1 to 2 mu M of a nucleic acid as a probe and a compound or a salt thereof, the compound being represented by the following formula: HS-L1-L2-R wherein L1 is a single bond or a C1-15 alkylene group; L2 is a single bond, nucleic acid, a polyethylene glycol group, -CO-NH-, or -NH-CO-; R is a hydroxyl group, an amino group, a ferrocenyl group, or a carboxyl group; provided that neither L1 nor L2 is a single bond, and incubating the composition in contact with a surface of the solid phase substrate. IMAGE