Apparatus for the primer extension reaction detection method pyrophosphate and measurement method, and to implement these methods

摘要

The present invention, the type of enzyme required is small, and extension reaction detection method of the primer, as well as for carrying out these methods and (PPi) quantitative measurement method pyrophosphate without requiring strict temperature control and to provide an apparatus and kit. PPi measurement method of the present invention, actinomycetes ^H that is inherent in the membrane ⁺ - by pyrophosphatase for ^(H + -PPase), by the action of PPi sample of unknown concentration, to analyze the transport of ^{H +} resulting , I will measure the PPi concentration in the sample. In addition, the extension reaction detection method of the primers of the present invention, H, which is allowed to act the unknown nucleic acid sample to be examined against actinomycetes H ⁺ -PPase that was inherent in the membrane, primer extension reaction is about whether or not occur, resulting ⁺ By analyzing the transport, it is determined the primer extension reaction is to whether it is for an unknown nucleic acid sample.