VACCINE DEVELOPMENT STRATEGY USING MICROGRAVITY CONDITIONS

摘要

Methods are provided herein for producing a vaccine, comprising culturing bacteria in microgravity. In some examples, the method includes culturing bacteria in microgravity, evaluating RNA expression, detecting an RNA that is over- or underexpressed during culture in microgravity, deleting the over- or underexpressed RNA in bacteria, and killing or attenuating the bacteria to produce a vaccine. In other examples, the method comprises culturing bacteria in microgravity, evaluating RNA expression, detecting a RNA that is over- or underexpressed during culture in microgravity, selecting bacteria that over- or underexpress the RNA, culturing the selected bacteria, and killing the bacteria to produce a vaccine. Vaccine compositions produced by the disclosed methods are also contemplated.