METHODS OF ISOLATING AND PURIFYING NUCLEIC ACID-BINDING BIOMOLECULES AND COMPOSITIONS INCLUDING SAME

摘要

The invention provides methods for isolating or purifying a biomolecule directly or indirectly bound to a region of interest of a nucleic acid in a cell, and methods for isolating or purifying a biomolecule that directly or indirectly binds to a region of interest of a nucleic acid in a cell. The invention further provides substantially cell free, isolated and purified biomolecule(s) that are fixed or cross-linked to a region of interest of a nucleic acid, which optionally reflects the interaction of the biomolecule(s) with the nucleic acid in the cell (e.g., in native chromatin) when fixed or cross-linked to the region of interest. Substantially cell free, isolated and purified biomolecules that are fixed or cross-linked to a region of interest of a nucleic acid can remain fixed or cross-linked to the region of interest of the nucleic acid when heated or treated with denaturing compounds or agents.