Hybridization Detection Method Using an Intercalator

摘要

To provide a novel and useful technique capable of achieving an improve S/N ratio in a hybridization detection technique using an intercalator. A method is provided wherein a hybridization is detected by measuring a fluorescence intensity from an intercalator I binding to a probe nucleic acid P and a complementary strand site of a target nucleic acid T. In the invention, a single-stranded target nucleic acid Ts, a single-stranded probe nucleic acid Ps forming no complementary strand and surplus nucleic acid moieties T₁₁, T₁₂ of a target nucleic acid T, which induce generation of noise fluorescence owing to the nonspecific binding of the intercalator I thereto, are digested and degraded with an enzyme having nuclease activity, such as a nucleolytic enzyme (nuclease) or the like.