Non-invasive real-time in vivo bioluminescence imaging of local Ca²+ dynamics in living organisms

摘要

A method for bioluminescence imaging in an animal is provided. The method comprises providing a whole animal containing a transcriptionally active nucleic acid sequence encoding a Ca²⁺-sensitive polypeptide, which comprises a chemiluminescent protein linked to a fluorescent protein; and monitoring photons emitted by the Ca²⁺-sensitive polypeptide. The Ca²⁺-sensitive polypeptide comprises aequorin protein covalently linked to a YFP (yellow fluorescent protein) or RFP (red fluorescent protein), and the link between the two proteins functions to allow luminescence by energy transfer between the two proteins. The photons are monitored from deep tissues of the animal.