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METHOD OF ISOTOPE LABELING AND DETERMINING PROTEIN SYNTHESIS, QUANTITATION AND PROTEIN EXPRESSION
METHOD OF ISOTOPE LABELING AND DETERMINING PROTEIN SYNTHESIS, QUANTITATION AND PROTEIN EXPRESSION
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机译:同位素标记和测定蛋白质的合成,定量和蛋白质表达的方法
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摘要
A method including isotope labeling of a newly synthesized protein in a sufficient quantity such that a newly synthesized protein spectra and the pre-existing protein spectra are sufficiently separated. A further method including determining a ratio of a new and a pre-existing protein from mass spectra obtained by using mass spectrometry. In this method a resultant spectrum may be presented as integrated peak heights for a corresponding mass to charge ratio in the “centroid” mode.
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