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NESTED PCR-BASED METHOD FOR SPECIFIC GENOTYPING OF THE FC GAMMA RECEPTOR IIIA GENE
NESTED PCR-BASED METHOD FOR SPECIFIC GENOTYPING OF THE FC GAMMA RECEPTOR IIIA GENE
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机译:基于嵌套PCR的FC GAMMA受体IIIA基因的特定基因分型方法
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摘要
The application describes a novel method for detection of a single nucleotide polymorphism, e.g., the 158 F/V polymorphism, in the FcγRIIIa gene using nested PCR to amplify large gene amplicons to aid in, e.g., genotyping applications. Thus, based on the results obtained in FcγRIIIa genotyping analysis, the present invention provides specific, efficient, reproducible, and accurate detection of such polymorphisms in genomic DNA.
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机译:该申请描述了一种新颖的方法,该方法用于检测FcγRIIIa基因中单核苷酸多态性(例如158 F / V多态性)的方法,该方法使用嵌套式PCR扩增大的基因扩增子,以帮助例如基因分型应用。因此,基于在FcγRIIIa基因分型分析中获得的结果,本发明提供了基因组DNA中这种多态性的特异性,有效,可再现和准确的检测。
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