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Method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor

机译:评估稻瘟病菌对鞘氨醇脱水酶抑制剂敏感性的方法

摘要

The present invention provides a method for assessing sensitivity of a rice blast fungus to a scytalone dehydratase inhibitor, comprising the steps of: (a) and (b), which (a) identifies an amino acid in an amino acid sequence of a scytalone dehydratase isolated from the rice blast fungus, where the amino acid corresponds to valine at position 75 of SEQ ID NO: 4; and (b) assesses the sensitivity of the rice blast fungus to the scytalone dehydratase inhibitor, where if the amino acid identified in step (a) is methionine, the sensitivity of the rice blast fungus to The scytalone dehydratase inhibitor is assessed to be lower than that of a wild-type rice blast fungus which endogenously produces the scytalone dehydratase of SEQ ID NO: 4.
机译:本发明提供了一种评估稻瘟病菌对双十一酮脱水酶抑制剂的敏感性的方法,其包括以下步骤:(a)和(b),其中(a)鉴定双十一酮脱水酶的氨基酸序列中的氨基酸。分离自稻瘟病真菌,其中氨基酸对应于SEQ ID NO:4的75位的缬氨酸; (b)评估稻瘟病菌对双胍类脱水酶抑制剂的敏感性,其中如果步骤(a)中鉴定的氨基酸是蛋氨酸,则稻瘟病菌对双胍类脱水酶抑制剂的敏感性被评估为低于内源性产生SEQ ID NO:4的双鞘甾醇脱水酶的野生型稻瘟病真菌的SNP。

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