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RNA polymerase III promoter, process for producing the same and method of using the same

机译:RNA聚合酶III启动子,其生产方法和使用方法

摘要

The present invention provides an RNA polymerase III promoter characterized in that a part of its sequence is substituted with a sequence capable of undergoing specific DNA recombination by Cre recombinase in such a manner functions of PSE and a TATA box are not impaired. This RNA polymerase III promoter can be used in a RNAi induction method with the condition that RNAi molecules to be used in analyzing gene function can be induced at an arbitrary point of time or only in a specific tissue (organ).
机译:本发明提供了一种RNA聚合酶III启动子,其特征在于其序列的一部分被能够通过Cre重组酶进行特异性DNA重组的序列取代,而不会损害PSE和TATA盒的功能。该RNA聚合酶III启动子可以用于RNAi诱导方法,其条件是可以在任意时间点或仅在特定组织(器官)中诱导用于分析基因功能的RNAi分子。

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