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Simultaneous anaerobic production of isoamyl acetate and succinic acid
Simultaneous anaerobic production of isoamyl acetate and succinic acid
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机译:乙酸异戊酯和琥珀酸同时厌氧生产
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摘要
In vivo method of producing esters from acetyle coA, such as isoamyl acetate and succinate, has been developed by producing null mutants in pathways that use acetyl coA and by overexpressing products that use NADH and in order to maintain the proper redox balance between NADH and NAD+. The method is exemplified with null mutations in ldhA, adhE, ackA-pta and overexpression of pyruvate carboxylase and alcohol acetyltransferase. This strain produces higher levels of both isoamyl acetate and succinate.
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