* modified Bacillus thuringiensis cry3bb polypeptide composition containing virus, Gene, vector, host Cell Transformed, using the polypeptide cry3bb * method to kill an insect, Beetle, method for Controlling a population of Beetles, method for producing u

摘要

A polypeptide cry3bb of Bacillus thuringiensis isolated modified to achieve Enhanced insecticidal activity against an insect insecticide or greater specificity determined includes the substitution, addition or deletion of at least one amino acid in the sequence of a cry3bb Native.A composition comprising an effective amount of the insecticide is described by their polypeptide sequences as well as the process for its production and a polynucleotide comprising a region of Sequence that encodes the polypeptide isolated.Furthermore we describe a Vector comprising the polynucleotide, a virus, a cell transformed; and a transgenic Plant comprising the polypeptide, its Progeny, Seed and Plant.A method to kill an insect Beetle, control the population of Beetles, a method for preparing a transgenic Plant resistant Beetles, and a method for preparing the seed of a Plant resistant to attacks of coleopteran insects.Claim 1: a polypeptide cry3bb * modified Bacillus thuringiensis, characterized in that it comprises one or more amino acids within the Loop alpha8 beta1, the primary sequence of the polypeptide cry3bb of SEQ ID no: 98 replaced by one or more amino acids are more hidrofobicida D, where one or more of these amino acid Replacements are selected from ser311 is replaced by alanine.Leucine or isoleucine, threonine, asn313 is replaced with arginine, histidine, Valine and threonine or glu317 is replaced by asparagine, alanine, Lysine or valine.